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Nanoprobes Inc aunps
Aunps, supplied by Nanoprobes Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aunps/aunps/10__1107_slash_s160057752600398x-73-11-15
Average 86 stars, based on 1 article reviews
aunps - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Synthesized:

Article Title: Gold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer.
Article Snippet: .. Supplementary Materials: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Figure S1: UV-Vis spectra of three different batches of the synthesized 35 nm AuNPs; Figure S2: UV-Vis spectra of three different batches of Au nanoprobes obtained with a oligonucleotide:AuNPs ratio of 1000; Figure S3: UV-Vis spectra analysis of the Au nanoprobe ratio 1000 incubated with MgCl2 at concentrations up to 25 mM; Figure S4: DNA concentration dependent effect of the AbsNon-Agg/AbsAgg ratio for 35 nm Au nanoprobes using three different targets: totally complementary (blue points and lines), deleted/noncomplementary (orange points and lines) and totally noncomplementary (purple points and lines) tested at different MgCl2 concentrations: 15 mM (A), 17.5mM (B), 20mM(C), 25 mM (D), 30 mM (E), 40mM (F) and 50mM(G); Figure S5: The bar graphs represent differences in AbsNon-Agg/AbsAgg ratios between complementary normal DNA (purple bar) and deleted/noncomplementary DNA (Orange lines) targets tested at different MgCl2 concentrations: at 15 mM (A) and 50 mM(B). ..

Article Title: Surface Functionalization of Citrate-Stabilized Gold Nanoparticles with Various Disease-Specific Nonthiolated Aptamers: RSM-Based Optimization for Multifactorial Disease Biomarker Detection
Article Snippet: .. The nanoprobes were synthesized based on optimized value, including 50 nm AuNPs and 0.5 μM concentration of BT. .. Raman spectroscopy was employed to analyze the interaction between the nanoprobes and the tau protein.

Incubation:

Article Title: Gold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer.
Article Snippet: .. Supplementary Materials: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Figure S1: UV-Vis spectra of three different batches of the synthesized 35 nm AuNPs; Figure S2: UV-Vis spectra of three different batches of Au nanoprobes obtained with a oligonucleotide:AuNPs ratio of 1000; Figure S3: UV-Vis spectra analysis of the Au nanoprobe ratio 1000 incubated with MgCl2 at concentrations up to 25 mM; Figure S4: DNA concentration dependent effect of the AbsNon-Agg/AbsAgg ratio for 35 nm Au nanoprobes using three different targets: totally complementary (blue points and lines), deleted/noncomplementary (orange points and lines) and totally noncomplementary (purple points and lines) tested at different MgCl2 concentrations: 15 mM (A), 17.5mM (B), 20mM(C), 25 mM (D), 30 mM (E), 40mM (F) and 50mM(G); Figure S5: The bar graphs represent differences in AbsNon-Agg/AbsAgg ratios between complementary normal DNA (purple bar) and deleted/noncomplementary DNA (Orange lines) targets tested at different MgCl2 concentrations: at 15 mM (A) and 50 mM(B). ..

Concentration Assay:

Article Title: Gold Nanoprobes for Detection of a Crucial EGFR Deletion for Early Diagnosis of Non-Small-Cell Lung Cancer.
Article Snippet: .. Supplementary Materials: The following supporting information can be downloaded at: www.mdpi.com/xxx/s1, Figure S1: UV-Vis spectra of three different batches of the synthesized 35 nm AuNPs; Figure S2: UV-Vis spectra of three different batches of Au nanoprobes obtained with a oligonucleotide:AuNPs ratio of 1000; Figure S3: UV-Vis spectra analysis of the Au nanoprobe ratio 1000 incubated with MgCl2 at concentrations up to 25 mM; Figure S4: DNA concentration dependent effect of the AbsNon-Agg/AbsAgg ratio for 35 nm Au nanoprobes using three different targets: totally complementary (blue points and lines), deleted/noncomplementary (orange points and lines) and totally noncomplementary (purple points and lines) tested at different MgCl2 concentrations: 15 mM (A), 17.5mM (B), 20mM(C), 25 mM (D), 30 mM (E), 40mM (F) and 50mM(G); Figure S5: The bar graphs represent differences in AbsNon-Agg/AbsAgg ratios between complementary normal DNA (purple bar) and deleted/noncomplementary DNA (Orange lines) targets tested at different MgCl2 concentrations: at 15 mM (A) and 50 mM(B). ..

Article Title: Surface Functionalization of Citrate-Stabilized Gold Nanoparticles with Various Disease-Specific Nonthiolated Aptamers: RSM-Based Optimization for Multifactorial Disease Biomarker Detection
Article Snippet: .. The nanoprobes were synthesized based on optimized value, including 50 nm AuNPs and 0.5 μM concentration of BT. .. Raman spectroscopy was employed to analyze the interaction between the nanoprobes and the tau protein.

Adsorption:

Article Title: Immunosensors made of polymer-infused porous paper for the non-invasive detection of airways cytokines trapped by porous face masks
Article Snippet: The diverse physicochemical properties of polymers make them perfect candidates for developing biosensor elements when combined with porous substrates.. For example, microfluidic channels, valves, reservoirs and biorecognition elements have been proposed that rely on filling the cavities of porous cellulose with different polymers.. Yet, highly concentrated polymers are often too viscous to dispense them with precision using conventional printing methods.

Zeta Potential Analyzer:

Article Title: Metallization of Targeted Protein Assemblies in Cell‐Derived Extracellular Matrix by Antibody‐Guided Biotemplating
Article Snippet: .. The AuNPs used to measure the zeta potential were obtained through electroless plating of Au on a 1.4 nm nanogold not conjugated with antibodies (Nanoprobes, #2025). (B) The generation of PtCl6 2– was verified by the appearance of peaks at wavelength of 200 and 260 nm. ..

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Nuclear Magnetic Resonance:

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Spectroscopy:

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Membrane:

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Real-time Polymerase Chain Reaction:

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Fluorescence:

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

Imaging:

Article Title: "Light-On" Fluorescent Nanoprobes for Monitoring Dynamic Distribution of Cellular Nucleolin During Pyroptosis.
Article Snippet: Nucleolin (NCL) is a multifunctional nuclear protein that plays significant roles in regulating physiological activities of the cells.. However, it remains a challenge to monitor the dynamic distribution and expression of nucleolin within living cells during cell stress processes directly.. Here, we designed “turn-on” fluorescent nanoprobes composed of specific AS1411 aptamer and nucleustargeting peptide on gold nanoparticles (AuNPs) to effectively capture and track the NCL distribution and expression during pyroptosis triggered by electrical stimulation (ES).

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

In Vitro:

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

In Vivo:

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

Transmission Assay:

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

Article Title: Simultaneous Down-Regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-Functionallized Gold Nanoprobes to Achieve Targeted Anti-Tumor Therapy.
Article Snippet: .. Characterization of AuNPs and Au Nanoprobes The size (~13 nm) and the spherical morphology of AuNPs were characterized by transmission electron microscopy (TEM) (Figure S1A). ..

Electron Microscopy:

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

Article Title: Simultaneous Down-Regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-Functionallized Gold Nanoprobes to Achieve Targeted Anti-Tumor Therapy.
Article Snippet: .. Characterization of AuNPs and Au Nanoprobes The size (~13 nm) and the spherical morphology of AuNPs were characterized by transmission electron microscopy (TEM) (Figure S1A). ..

Transmission Electron Microscopy:

Article Title: Fluorescent glyco-gold nanocluster induced EGFR mediated targeting of cancer cells.
Article Snippet: A lot of attention has been focused on the functionalization of carbohydrate ligands on specific sizes and shapes of gold nanoparticles (AuNPs), where ultrasmall fluorescent AuNPs have not been well explored for direct imaging.. Herein, we have engineered fluorescent gold nanoclusters with sulfated oligo-iduronic acid ligands (I34), which strongly bind to the HB-EGF receptor over FGF2, and regulate EGF receptor-mediated cancer cell homing in both twoand three-dimensional (2D and 3D) cell culture systems.. These results offer a new practical and direct imaging tool for carbohydrate research.

Article Title: Simultaneous Down-Regulation of Intracellular MicroRNA-21 and hTERT mRNA Using AS1411-Functionallized Gold Nanoprobes to Achieve Targeted Anti-Tumor Therapy.
Article Snippet: .. Characterization of AuNPs and Au Nanoprobes The size (~13 nm) and the spherical morphology of AuNPs were characterized by transmission electron microscopy (TEM) (Figure S1A). ..



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Preparation and characterization of LL37-AuNPs@PUE. Note: ( A) Schematic representation of the synthesis of LL37-AuNPs@PUE; ( B ) UV-Vis absorption spectra of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE; ( C ) Representative TEM images of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE (scale bar = 20 nm); ( D ) Particle size distribution of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE measured by DLS; ( E ) Zeta potential of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE measured by DLS; ( F ) In vitro release profiles of PUE from AuNPs@PUE and LL37-AuNPs@PUE; ( G) Particle size stability of AuNPs@PUE and LL37-AuNPs@PUE in 10% FBS over 72 h; ( H ) Standard curve of PUE concentration versus absorbance at 250 nm wavelength; ( I ) Drug loading content and EE of PUE in LL37-AuNPs@PUE; ( J ) Changes in particle size of LL37-AuNPs@PUE over one week; ( K ) Changes in PDI of LL37-AuNPs@PUE over one week. All experiments were performed in triplicate. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Preparation and characterization of LL37-AuNPs@PUE. Note: ( A) Schematic representation of the synthesis of LL37-AuNPs@PUE; ( B ) UV-Vis absorption spectra of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE; ( C ) Representative TEM images of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE (scale bar = 20 nm); ( D ) Particle size distribution of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE measured by DLS; ( E ) Zeta potential of AuNPs, LL37-AuNPs, AuNPs@PUE, and LL37-AuNPs@PUE measured by DLS; ( F ) In vitro release profiles of PUE from AuNPs@PUE and LL37-AuNPs@PUE; ( G) Particle size stability of AuNPs@PUE and LL37-AuNPs@PUE in 10% FBS over 72 h; ( H ) Standard curve of PUE concentration versus absorbance at 250 nm wavelength; ( I ) Drug loading content and EE of PUE in LL37-AuNPs@PUE; ( J ) Changes in particle size of LL37-AuNPs@PUE over one week; ( K ) Changes in PDI of LL37-AuNPs@PUE over one week. All experiments were performed in triplicate. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: Zeta Potential Analyzer, In Vitro, Concentration Assay, Standard Deviation

In vitro antimicrobial evaluation of LL37-AuNPs@PUE. Note: ( A ) Schematic of co-incubation of different concentrations of PUE, AuNPs@PUE, or LL37-AuNPs@PUE with Pg for specified times; ( B ) Quantification of bacterial survival after 24 h of co-incubation with different concentrations of PUE, AuNPs@PUE, or LL37-AuNPs@PUE. Survival was normalized to the Control group to evaluate the antibacterial efficacy of each formulation; ( C ) Quantification of bacterial survival after varying co-incubation times with equal concentrations of PUE, AuNPs@PUE, or LL37-AuNPs@PUE. Survival was normalized to the Control group to evaluate the antibacterial efficacy of each formulation; ( D) Representative images of live/dead staining in different treatment groups (scale bar = 25 μm) and the ratio of green (live) to red (dead) fluorescence intensity; ( E ) Schematic of PUE, AuNPs@PUE, or LL37-AuNPs@PUE treatment of Pg biofilms; ( F ) OD at 595 nm of various treatment groups after crystal violet staining; ( G ) Representative SEM images of Pg in each treatment group (scale bar = 2 μm); ( H ) Levels of β-galactosidase release from Pg cells in each treatment group; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 between groups; all experiments were repeated three times. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: In vitro antimicrobial evaluation of LL37-AuNPs@PUE. Note: ( A ) Schematic of co-incubation of different concentrations of PUE, AuNPs@PUE, or LL37-AuNPs@PUE with Pg for specified times; ( B ) Quantification of bacterial survival after 24 h of co-incubation with different concentrations of PUE, AuNPs@PUE, or LL37-AuNPs@PUE. Survival was normalized to the Control group to evaluate the antibacterial efficacy of each formulation; ( C ) Quantification of bacterial survival after varying co-incubation times with equal concentrations of PUE, AuNPs@PUE, or LL37-AuNPs@PUE. Survival was normalized to the Control group to evaluate the antibacterial efficacy of each formulation; ( D) Representative images of live/dead staining in different treatment groups (scale bar = 25 μm) and the ratio of green (live) to red (dead) fluorescence intensity; ( E ) Schematic of PUE, AuNPs@PUE, or LL37-AuNPs@PUE treatment of Pg biofilms; ( F ) OD at 595 nm of various treatment groups after crystal violet staining; ( G ) Representative SEM images of Pg in each treatment group (scale bar = 2 μm); ( H ) Levels of β-galactosidase release from Pg cells in each treatment group; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 between groups; all experiments were repeated three times. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: In Vitro, Incubation, Control, Formulation, Staining, Fluorescence, Standard Deviation

Impact of LL37-AuNPs@PUE on HAECs cellular phenotype post-Pg infection. Note: ( A ) Schematic of HAECs treated with Pg in the presence of free PUE, AuNPs@PUE, LL37-AuNP, or LL37-AuNPs@PUE; ( B ) Cell viability of each group determined by CCK-8 assay; ( C ) Representative images of EdU staining in each group (scale bar = 100 μm) and quantification of EdU positive cells percentage; ( D ) Representative images of cell migration in each group measured by Transwell assay (scale bar = 100 μm) and quantification of migrated cells; ( E ) Apoptosis rate in each group assessed by flow cytometry; ( F ) Expression of inflammatory markers TNF-α, IL-1β, and IL-6 in each group detected by RT-qPCR; ( G ) Levels of TNF-α, IL-1β, and IL-6 in the supernatant of cultured HAECs from each group measured by ELISA; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001; all experiments were repeated three times. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Impact of LL37-AuNPs@PUE on HAECs cellular phenotype post-Pg infection. Note: ( A ) Schematic of HAECs treated with Pg in the presence of free PUE, AuNPs@PUE, LL37-AuNP, or LL37-AuNPs@PUE; ( B ) Cell viability of each group determined by CCK-8 assay; ( C ) Representative images of EdU staining in each group (scale bar = 100 μm) and quantification of EdU positive cells percentage; ( D ) Representative images of cell migration in each group measured by Transwell assay (scale bar = 100 μm) and quantification of migrated cells; ( E ) Apoptosis rate in each group assessed by flow cytometry; ( F ) Expression of inflammatory markers TNF-α, IL-1β, and IL-6 in each group detected by RT-qPCR; ( G ) Levels of TNF-α, IL-1β, and IL-6 in the supernatant of cultured HAECs from each group measured by ELISA; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001; all experiments were repeated three times. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: Infection, CCK-8 Assay, Staining, Migration, Transwell Assay, Flow Cytometry, Expressing, Quantitative RT-PCR, Cell Culture, Enzyme-linked Immunosorbent Assay, Standard Deviation

Effects of LL37-AuNPs@PUE on atherosclerosis and inflammatory phenotype in periodontitis mice. Note: ( A ) Representative images of Oil Red O staining of aorta from each group (n=6) and quantification of plaque area percentage; ( B ) Representative images of Oil Red O stained aortic sections from each group (n=6) (scale bar = 100 μm) and quantification of plaque area percentage; ( C ) Representative images of H&E stained aortic sections from each group (n=6) (scale bar = 100 μm) and quantification of necrotic core percentage; ( D ) Immunohistochemical staining for inflammatory markers TNF-α, IL-1β, and IL-6 in aortic sections from each group (n=6) (scale bar = 50 μm), quantification of positive staining area percentage; ( E ) Levels of inflammatory markers TNF-α, IL-1β, and IL-6 in the serum of each group (n=6); * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Effects of LL37-AuNPs@PUE on atherosclerosis and inflammatory phenotype in periodontitis mice. Note: ( A ) Representative images of Oil Red O staining of aorta from each group (n=6) and quantification of plaque area percentage; ( B ) Representative images of Oil Red O stained aortic sections from each group (n=6) (scale bar = 100 μm) and quantification of plaque area percentage; ( C ) Representative images of H&E stained aortic sections from each group (n=6) (scale bar = 100 μm) and quantification of necrotic core percentage; ( D ) Immunohistochemical staining for inflammatory markers TNF-α, IL-1β, and IL-6 in aortic sections from each group (n=6) (scale bar = 50 μm), quantification of positive staining area percentage; ( E ) Levels of inflammatory markers TNF-α, IL-1β, and IL-6 in the serum of each group (n=6); * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: Staining, Immunohistochemical staining, Standard Deviation

Impact of ENPP2 overexpression on endothelial inflammation induced by Pg. Note: ( A ) Schematic of ENPP2-OE transfection; ( B ) RT-qPCR analysis of ENPP2 expression in various HAECs groups; ( C ) Western Blot analysis of ENPP2 expression in different HAECs groups; ( D ) Schematic of HAECs treated with Pg post-NC-OE or ENPP2-OE transfection in the presence or absence of LL37-AuNPs@PUE; ( E ) RT-qPCR analysis of inflammatory markers TNF-α, IL-1β, and IL-6 in HAECs; ( F ) ELISA measurement of TNF-α, IL-1β, and IL-6 levels in supernatants from cultured HAECs; ( G ) Western Blot analysis and quantification of PPARG expression in HAECs; ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, all experiments were repeated three times. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Impact of ENPP2 overexpression on endothelial inflammation induced by Pg. Note: ( A ) Schematic of ENPP2-OE transfection; ( B ) RT-qPCR analysis of ENPP2 expression in various HAECs groups; ( C ) Western Blot analysis of ENPP2 expression in different HAECs groups; ( D ) Schematic of HAECs treated with Pg post-NC-OE or ENPP2-OE transfection in the presence or absence of LL37-AuNPs@PUE; ( E ) RT-qPCR analysis of inflammatory markers TNF-α, IL-1β, and IL-6 in HAECs; ( F ) ELISA measurement of TNF-α, IL-1β, and IL-6 levels in supernatants from cultured HAECs; ( G ) Western Blot analysis and quantification of PPARG expression in HAECs; ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001, all experiments were repeated three times. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Cell Culture, Standard Deviation

Regulation of atherosclerosis in periodontitis mice by LL37-AuNPs@PUE via the ENPP2/PPARG pathway. Note: ( A ) Western Blot analysis and protein quantification of ENPP2 and PPARG in aortic tissue from each group ( n =6); ( B ) Representative images of Oil Red O staining of aortas from each group ( n =6) and quantification of plaque area percentage; ( C ) Representative images of aortic slices stained with Oil Red O (scale bar=100 μm) and quantification of plaque area percentage; ( D ) Representative images of aortic slices stained with H&E (scale bar=100 μm) and quantification of necrotic core percentage; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Regulation of atherosclerosis in periodontitis mice by LL37-AuNPs@PUE via the ENPP2/PPARG pathway. Note: ( A ) Western Blot analysis and protein quantification of ENPP2 and PPARG in aortic tissue from each group ( n =6); ( B ) Representative images of Oil Red O staining of aortas from each group ( n =6) and quantification of plaque area percentage; ( C ) Representative images of aortic slices stained with Oil Red O (scale bar=100 μm) and quantification of plaque area percentage; ( D ) Representative images of aortic slices stained with H&E (scale bar=100 μm) and quantification of necrotic core percentage; * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: Western Blot, Staining, Standard Deviation

Influence of LL37-AuNPs@PUE on vascular inflammation and lipid levels in periodontitis mice via the ENPP2/PPARG pathway. Note: ( A ) Representative immunohistochemical staining images of inflammatory markers TNF-α, IL-1β, and IL-6 in aortic slices from each group ( n =6), scale bar = 50 μm, quantification of positive staining area percentage; ( B ) Levels of inflammatory markers TNF-α, IL-1β, and IL-6 in serum from each group ( n =6); ( C ) Lipid profile levels (TG, TC, LDL-C, HDL-C) in serum from each group ( n =6); * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All quantitative data are presented as mean ± standard deviation

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Influence of LL37-AuNPs@PUE on vascular inflammation and lipid levels in periodontitis mice via the ENPP2/PPARG pathway. Note: ( A ) Representative immunohistochemical staining images of inflammatory markers TNF-α, IL-1β, and IL-6 in aortic slices from each group ( n =6), scale bar = 50 μm, quantification of positive staining area percentage; ( B ) Levels of inflammatory markers TNF-α, IL-1β, and IL-6 in serum from each group ( n =6); ( C ) Lipid profile levels (TG, TC, LDL-C, HDL-C) in serum from each group ( n =6); * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001. All quantitative data are presented as mean ± standard deviation

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: Immunohistochemical staining, Staining, Standard Deviation

Schematic of the molecular mechanism by which LL37-AuNPs@PUE prevents atherosclerosis in periodontitis mice

Journal: Journal of Nanobiotechnology

Article Title: Nanocomposite LL37-gold nanoparticles with puerarin for periodontitis-linked atherosclerosis

doi: 10.1186/s12951-026-04204-9

Figure Lengend Snippet: Schematic of the molecular mechanism by which LL37-AuNPs@PUE prevents atherosclerosis in periodontitis mice

Article Snippet: To obtain PUE-loaded AuNPs (AuNPs@PUE), PUE (MCE, HY-N0145) was dissolved in DMSO (1.5 mL, 0.2 mg/mL, pH 7.4) and mixed with AuNPs.

Techniques: